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| tag | His |
| host species | 2019-nCoV |
| accession | YP_009725309.1 |
| synonym | SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase |
| background | The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development. |
| description | Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus. |
| source | E.coli |
| AA sequence | Ala1-Gln527 |
| molecular weight | 62.9kDa. AP Mol Mass: 60kDa, reducing conditions. |
| purity | >85% as determined by reducing SDS-PAGE. |
| formulation | Recombinant 2019-nCoV Guanine-N7 methyltransferase was Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4. |
| Sensitivity | For Research Use Only |
| Detection Limit | Store at < -20°C, stable for 6 months after receipt. Please Avoid repeated freeze-thaw cycles. |
