Recombinant 2019-nCoV 3C-like Proteinase (His Tag)

Collections: Coronaviruses, Featured viral antigens molecules, Recombinant proteins, Viral antigen

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Product Overview

Tag	His
Host Species	2019-nCoV
Accession	YP_009725301.1
Synonym	M Proteinase; 3CL Proteinase
Background	The viral main proteinase (M pro , also called 3CL pro ), which controls the activities of the coronavirus replication complex. It functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replication and is considered as an appealing target for designing anti-SARS agents.
Description	Recombinant 2019-nCoV 3C-like Proteinase is expressed in E.coli and the target gene encoding Ser1-Gln306 is fused with a 6His tag at the N-terminus.
Source	E.coli
AA Sequence	Ser1-Gln306
Molecular Weight	36.1kDa AP Mol Mass: 33kDa, reducing condiï¿½ons.
Purity	> 95 % as determined by SDS-PAGE.
Formulation	Recombinant 2019-nCoV 3C-like Proteinase is supplied as a 0.2 μm filtered solution of PBS, 1mM EDTA, 10% Glycerol, pH 7.4.
Stability	Recombinant 2019-nCoV 3C-like Proteinase is stable for up to 1 year from date of receipt at -20°C to -80°C.
Usage	For Research Use Only
Storage	Store Recombinant 2019-nCoV 3C-like Proteinase under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.