Recombinant Rhesus PCSK9 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3678
Recombinant Rhesus PCSK9 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-3678
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Rhesus |
Accession | NP_001106130.1 |
Background | Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons. References |
Description | A DNA sequence encoding the Rhesus PCSK9 (NP_001106130.1) (Met 1-Gln 692) precursor was expressed, fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gln 31 |
AA Sequence | Met 1-Gln 692 |
Molecular Weight | The recombinant Rhesus PCSK9 consists of 673 a.a. and predicts a molecular mass of 72.7 kDa. As a result of glycosylation and proteolytic digestion, it migrates as doublet with apparent molecular mass of 20 kDa and 62 kDa corresponding to the pro domain and mature form respectively in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its ability to bind biotinylated human LDLR in a functional ELISA.2. Measured by its ability to bind biotinylated mouse LDLR in a functional ELISA. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |