Recombinant Rhesus Ephrin A5 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1869
Recombinant Rhesus Ephrin A5 Protein (Fc Tag)

Recombinant Rhesus Ephrin A5 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1869
Catalog No.: BLPSN-1869

Product Overview

Tag Fc
Host Species Rhesus
Accession NP_001252657.1
Background Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Description A DNA sequence encoding the rhesus EFNA5 (NP_001252657.1) (Met 1-Asn 203) was fused with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Gln 21
AA Sequence Met 1-Asn 203
Molecular Weight The recombinant rhesus EFNA5 consists of 424 a.a. and has a calculated molecular mass of 48.2 kDa.
Purity >92% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its binding ability in a functional ELISA.Immobilized Rhesus EFNA5-Fc at 10 ug/mL (100 ul/well) can bind biotinylated Rhesus EPHA4. The EC50 of biotinylated Rhesus EPHA4 is 9.3-21.6 ng/mL.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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