Recombinant Rat TWEAK Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4701
Recombinant Rat TWEAK Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4701
Product Overview
| Tag | Fc |
| Host Species | Rat |
| Accession | NP_001001513.2 |
| Description | A DNA sequence encoding the rat TNFSF12 (Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu |
| AA Sequence | Arg105-His249 |
| Molecular Weight | The recombinant rat TNFSF12 comprises 405 a.a. and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 5-20 ng/mL. 2. Immobilized Fc-rat TNFSF12 at 10 ug/ml (100 ul/well) can bind biotinylated human Fc-TNFRSF12A, The EC50 of biotinylated human Fc-TNFRSF12A is 0.12-0.28 ug/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 . |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
