Recombinant Rat TWEAK Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4701
Recombinant Rat TWEAK Protein (Fc Tag)

Recombinant Rat TWEAK Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-4701

Product Overview

Tag Fc
Host Species Rat
Accession NP_001001513.2
Description A DNA sequence encoding the rat TNFSF12 (Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus.
Source HEK293
Predicted N Terminal Glu
AA Sequence Arg105-His249
Molecular Weight The recombinant rat TNFSF12 comprises 405 a.a. and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity >85% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 5-20 ng/mL. 2. Immobilized Fc-rat TNFSF12 at 10 ug/ml (100 ul/well) can bind biotinylated human Fc-TNFRSF12A, The EC50 of biotinylated human Fc-TNFRSF12A is 0.12-0.28 ug/ml.
Formulation Lyophilized from sterile PBS, pH 7.4 .
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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