Recombinant Rat TrkB Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4668
Recombinant Rat TrkB Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4668
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Rat |
| Accession | Q63604 |
| Background | TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the rat NTRK2 (Q63604-Isoform T1) (Met1-His429) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Cys 32 |
| AA Sequence | Met1-His429 |
| Molecular Weight | The recombinant rat NTRK2 comprises 409 a.a. and predicts a molecular mass of 47.1 kDa. The apparent molecular mass of the recombinant protein is approximately 67 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA.Immobilized rat NTRK2-His at 10 ug/ml (100 ul/well) can bind biotinylated mouse BDNF, The EC50 of biotinylated mouse BDNF is 12.2-28.6 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
