Recombinant Rat Transferrin Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4627
Recombinant Rat Transferrin Protein (His Tag)

Recombinant Rat Transferrin Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-4627
Catalog No.: BLPSN-4627

Product Overview

Tag His
Host Species Rat
Accession NP_001013128.1
Background Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins which bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe3+ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.
Description A DNA sequence encoding the rat TF (NP_001013128.1) (Met1-Ser698) was expressed with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Val 20
AA Sequence Met1-Ser698
Molecular Weight The recombinant rat TF comprises 690 a.a. and predicts a molecular mass of 76 kDa. The apparent molecular mass of the recombinant protein is approximately 89 kDa in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.02-1 ug/mL.
Formulation Lyophilized from sterile PBS, pH 7.2..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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