Recombinant Rat GRO beta / CXCL2 Protein

Name: Recombinant Rat GRO beta / CXCL2 Protein
Brand: Beta LifeScience
SKU: BLPSN-2336
Availability: InStock

Collections: Chemokines and receptors, Cytokines and growth factors, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Rat
Accession	NP_446099.1
Background	Chemokine (C-X-C motif) ligand 2 (CXCL2), also called macrophage inflammatory protein 2 (MIP-2), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2), is a small cytokine belonging to the CXC chemokine family. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone, where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for CXCL2/MIP-2 or in the presence of a blocking Ab to CXCL2/MIP-2, peripheral tolerance is prevented, and Ag-specific T regulatory cells are not generated. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone, where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for MIP-2 (as in CXCR2-deficient mice) or in the presence of a blocking Ab to MIP-2, peripheral tolerance is prevented, and Ag-specific T regulatory cells are not generated. Understanding the regulation of lymphocyte traffic during tolerance induction may lead to novel therapies for autoimmunity, graft acceptance, and tumor rejection. Several studies have implicated the CXCL2 chemokine as a mediator in the development of sepsis. CXCL2/MIP-2 also plays a major role in mediating the neutrophilic inflammatory response of the rodent lung to particles such as quartz, crocidolite asbestos, as well as high doses of other relative innocuous dusts such as titanium dioxide.
Description	A DNA sequence encoding the rat Cxcl2 (NP_446099.1) (Ser32-Asn100) was expressed with two additional amino acids (Gly & Pro ) at the N-terminus.
Source	E.coli
Predicted N Terminal	Ser 69
AA Sequence	Ser32-Asn100
Molecular Weight	The recombinant rat Cxcl2 consists 69 a.a. and predicts a molecular mass of 7.6 kDa.
Purity	>85% as determined by SDS-PAGE.
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Subcellular Location	Secreted.
Protein Families	Intercrine alpha (chemokine CxC) family
Database References	KEGG: rno:114105 STRING: 10116.ENSRNOP00000003745 UniGene: Rn.10230
Tissue Specificity	At least expressed in the lung and trachea.

Gene Functions References

this study shows elevated expression of CINC-3 at the site of inflammation as well as their significant reflection in the circulation, thereby suggesting their frontline role in carrageenan-induced acute inflammation PMID: 27967265
rutin is a potential protective agent in spinal cord injury enhancing the neurotrophic effect by inhibiting the expression of MIP-2 and activation of MMP-9, and downregulating the expression of p-Akt. PMID: 26502930
The rat spiral ligament fibrocytes were found to release CXCL2 in response to nontypeable H. influenzae via activation of c-Jun, leading to the recruitment of polymorphonuclear cells to the cochlea. PMID: 22379036
CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats. PMID: 20034917
Results identify FQHPSFI peptide may be used for the modulation of lipopolysaccharide-stimulated MIP-2 production in alveolar macrophagess. PMID: 20186460
chemokine production by rat alveolar macrophages is inhibited by taurine chloramine PMID: 11716962
Intratracheal LPS induced a significant increase in MIP-2 in bronchoalveolar lavage (BAL) fluid with no detectable MIP-2 in the plasma. PMID: 11837784
demonstrated that the beta-glucan component of the Pneumocystis carinii cell wall is able to stimulate alveolar epithelial cells to produce MIP-2 PMID: 11906040
role for the nitric oxide pathway in the overproduction of pro-inflammatory mediators IL-6 and MIP-2 during GBS-induced lung inflammation. PMID: 12661899
junctional epithelium cells produced MIP-2 and CINC-2 in response to LPS stimulation, suggesting that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation PMID: 15045510
macrophage inflammatory protein 2 mediates platelet-activating factor-induced intestinal injury PMID: 15319184
no significant differences were detected for MIP-2 levels in diabetic rats when compared with non-diabetic rats after hypoxia PMID: 16132691
Following cecal ligation and puncture Cxcl2 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
MIP-2/CXCL2 and MCP-1/CCL2 are increased after injury, and neurons appear to be the source of this expression. Chemokine expression was selectively inhibited by dexamethasone. PMID: 19210118

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.