Recombinant Rat gp130 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2305
Recombinant Rat gp130 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2305
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&Fc |
Host Species | Rat |
Accession | NP_001008725.2 |
Background | Glycoprotein 13 (also known as gp13, IL6ST, IL6-beta or CD13) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD13/gp13 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD13/gp13 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD13/gp13 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp13 for initiating signal transmission. CD13/gp13 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD13/gp13 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events. |
Description | A DNA sequence encoding the rat IL6ST (NP_001008725.2) extracellular domain (Met 4-Glu 618) was fused with the C-terminal His-tagged Fc region of mouse IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gln 23 |
AA Sequence | Met 4-Glu 618 |
Molecular Weight | The recombinant rat IL6ST/Fc is a disulfide-linked homodimer. The reduced monomer comprises 839 a.a. and predicts a molecular mass of 94.3 kDa. The apparent molecular mass of the rat IL6ST/Fc monomer is approximately 130 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to inhibit the IL6 Ralpha enhancement of IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.015-0.075 ug/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |