Name: Recombinant Rat ErbB4 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1911
Availability: InStock

Catalog No.: BLPSN-1911

Product Overview

Tag	His
Host Species	Rat
Accession	AAQ77349.1
Synonym	ERBB4, ALS19, HER4, p180erbB4, erb-b2 receptor tyrosine kinase 4
Background	Receptor tyrosine-protein kinase erbB-4 is an enzyme that in humans is encoded by the ERBB4 gene. Alternatively spliced variants that encode different protein isoforms have been described; however, not all variants have been fully characterized. Receptor tyrosine-protein kinase erbB-4 is a receptor tyrosine kinase that is a member of the epidermal growth factor receptor family. ERBB4 is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins-2 and -3, heparin-binding EGF-like growth factor and betacellulin. Ligand binding induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment.
Description	A DNA sequence encoding the rat ERBB4 (Met1-Pro651) was expressed with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Gln 26
AA Sequence	Met1-Pro651
Molecular Weight	The recombinant rat ERBB4 comprises 637 amino acids and predicts a molecular mass of 71.3 kDa. The apparent molecular mass of the recombinant protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized rat ERBB4-His at 10 ug/ml (100 ul/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.68-1.6 ug/ml.
Formulation	Recombinant Rat ErbB4 Protein (His Tag) was lyophilized from sterile PBS, pH 7.4.
Stability	Recombinant Rat ErbB4 protein is stable up to 1 year at -70°C
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.