Name: Recombinant Rat Ephrin A5 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1868
Availability: InStock

Recombinant Rat Ephrin A5 Protein (His Tag)

Catalog No.: BLPSN-1868

Product Overview

Tag	His
Host Species	Rat
Accession	P97605
Background	Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Description	A DNA sequence encoding the rat EFNA5 (P97605) (Met 1-Glu 202), without the pro peptide, was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Gln 21
AA Sequence	Met 1-Glu 202
Molecular Weight	The recombinant rat EFNA5 comprises 193 a.a. and predicts a molecular mass of 22.5 kDa. The apparent molecular mass of the ratEFNA5 is approximately 27 kDa in SDS-PAGE under reducing conditions.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA.Immobilized rat EFNA5-His at 10 ug/ml (100 ul/well) can bind rat EPHA3-Fc, The EC50 of rat EPHA3-Fc is 12.1-28.3 ng/ml.
Formulation	Lyophilized from sterile PBS, pH 7.5.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.