Recombinant Rat EphA3 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1806
Recombinant Rat EphA3 Protein (His Tag)

Recombinant Rat EphA3 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1806
Catalog No.: BLPSN-1806

Product Overview

Tag His
Host Species Rat
Accession G3V9D6
Background EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description A DNA sequence encoding the rat EPHA3 (EDL75897.1) (Met1-His541) was expressed with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Glu 21
AA Sequence Met1-His541
Molecular Weight The recombinant rat EPHA3 comprises 532 a.a. and predicts a molecular mass of 61.2 kDa. The apparent molecular mass of the recombinant protein is approximately 70.5 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its binding ability in a functional ELISA.Immobilized rat EPHA3-His at 10 ug/ml (100 ul /well) can bind rat EFNA5-Fc, The EC50 of ratEFNA5-Fc is 9-20ng/ml.
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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