Recombinant Rat DDR2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1579
Recombinant Rat DDR2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1579
Catalog No.: BLPSN-1579
Product Overview
| Tag | His |
| Host Species | Rat |
| Accession | B1WC09 |
| Background | Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the rat DDR2 (B1WC09) (Met1-Arg399) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gln 24 |
| AA Sequence | Met1-Arg399 |
| Molecular Weight | The recombinant rat DDR2 comprises 387 a.a. and predicts a molecular mass of 43.9 kDa. The apparent molecular mass of the recombinant protein is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
