Recombinant Rat Cystatin C Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1532
Recombinant Rat Cystatin C Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1532
Catalog No.: BLPSN-1532
Product Overview
| Tag | His |
| Host Species | Rat |
| Accession | P14841 |
| Background | Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 12 amino acid residues, with a molecular mass of 13,343-13,359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better diagnostic accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression. |
| Description | A DNA sequence encoding the rat CST3 (P14841-1) (Met 1-Ala 140) was fused with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gly 21 |
| AA Sequence | Met 1-Ala 140 |
| Molecular Weight | The recombinant rat CST3 comprises 131 a.a. and predicts a molecular mass of 14.7 kDa. The apparent molecular mass of the rat CST3 is approximately 18-25 kDa in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM HEPES, 150mM NaCl, pH 7.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
