Recombinant Rat ART4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0272
Recombinant Rat ART4 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0272
Catalog No.: BLPSN-0272
Product Overview
| Tag | His |
| Host Species | Rat |
| Accession | NP_001166980.1 |
| Background | ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. |
| Description | A DNA sequence encoding the rat ART4 (Met1-Lys269) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gly 30 |
| AA Sequence | Met1-Lys269 |
| Molecular Weight | The recombinant rat ART4 comprises 251 a.a. and predicts a molecular mass of 28.4 kDa. The apparent molecular mass of the recombinant protein is approximately 36-41 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
