Recombinant Mouse WIF1 Protein
Beta LifeScience
SKU/CAT #: BL-4359PS
Recombinant Mouse WIF1 Protein
Beta LifeScience
SKU/CAT #: BL-4359PS
Catalog No.: BL-4359PS
Product Overview
| Tag | His |
| Host Species | Mouse |
| Synonym | Wntinhibitory factor 1, WIF-1, Wif1. |
| Background | WIF1 binds to wnt proteins and inhibits their activities. WIF1 plays a role in mesoderm segmentation. WNT proteins are extracellular signaling molecules that take part in the control of embryonic development & cancer. WIF1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. WIF1 takes part in mesoderm segmentation. WIF1 protein is found to be present in fish, amphibia and mammals. WIF1 is a recurrent target in human salivary gland oncogenesis. Downregulation of WIF1 takes part in the development and progression of pleomorphic adenomas. WIF1 is a tumor suppressor, specifically in nonfunctioning pituitary tumors. |
| Description | WIF1 expressed in Sf9 Insect cells is a single,glycosylated polypeptide chain containing 359a.a. (29-379a.a.) andhaving a molecular weight of 39.4kDa. (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).WIF1 is expressed with an 8a.a. His tag atC-Terminus and purified by unique purification methods. |
| Source | Sf9 |
| AA Sequence | GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMPAIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVAAFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPHCEKALCIPRC MNGGLCVTPGFCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKPVCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH. |
| Purity | >90.0% asdetermined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | WIF1protein solution (1mg/ml) contains 20mM MES (pH5.5), 1mM DTT, 1mM PMSF and 30%glycerol. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
