Recombinant Mouse Vitamin D Binding Protein (His tag)

Beta LifeScience SKU/CAT #: BLA-10165P

Recombinant Mouse Vitamin D Binding Protein (His tag)

Beta LifeScience SKU/CAT #: BLA-10165P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Host Species Mouse
Accession P21614
Synonym DBP DBP/GC GC Gc globulin Gc-globulin GRD3 Group specific component Group specific component vitamin D binding protein Group-specific component hDBP VDB VDBG VDBP Vitamin D binding alpha globulin Vitamin D-binding protein VTDB_HUMAN
Description Recombinant Mouse Vitamin D Binding Protein (His tag) was expressed in Yeast. It is a Full length protein
Source Yeast
AA Sequence LERGRDYEKDKVCNELAMLGKEDFRSLSLILYSRKFSSSTFEQVNQLVKE VVSLTEECCAEGADPTCYDTRTSELSVKSCESDAPFPVHPGTPECCTKEG LERKLCMAALSHQPQEFPTYVEPTNDEICEAFRRDPKGFADQFLYEYSSN YGQAPLPLLVAYTKNYLSMVGSCCTSANPTVCFVKERLQMKHLSLLTTMS NRVCSQYAAYGKEKSRLSHLIKLAQKVPTANLENVLPLAEDFTEILSRCC ESTSEDCMASELPEHTIKICQNLSKKNSKFEECCQENTPMNIFMCTYFMP AAEPLQLPAIKLPTGKDLCGQSTTQAMDQYTFELSRRTQVPEVFLSKVLE PTLKTLRECCDTQDSVACFSTQSPLLKRQLTSFIEKGQEMCADYSENTFT EYKKKLAERLRTKTPNTSPAELKDMVEKHSDFASKCCSINSPPLYCSSQI DAEMIDTLQS
Molecular Weight 54 kDa including tags
Purity >85% SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Liquid Solution
Stability The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution See related COA
Unit Definition For Research Use Only
Storage Buffer Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Subcellular Location Secreted.
Protein Families ALB/AFP/VDB family
Database References

Gene Functions References

  1. results show DBP is a significant chemotactic cofactor in vivo and not specific for C5a, suggesting that this ubiquitous plasma protein may have a more significant role in neutrophil recruitment than previously recognized PMID: 23752613
  2. Effects of estradiol on the endocytic transport of vitamin D carrier protein in hepatocytes. PMID: 23416408
  3. Data suggest that whereas DBP is important to total circulating 1,25(OH)(2)D(3) and sequesters extracellular levels of this hormone both in vivo and in vitro, the binding protein does not influence the hormone's biologically active pool. PMID: 18372326

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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