Recombinant Mouse UCHL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4722
Recombinant Mouse UCHL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4722
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | Q9R0P9 |
Synonym | AW822034, C88048, gad, PGP9.5, R75593, UCH-L1, UCHL-1 |
Background | Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons. |
Description | A DNA sequence encoding the mouse UCHL1 (Q9R0P9) (Gln 2-Ala 223) was expressed, with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Gln 2-Ala 223 |
Molecular Weight | The recombinant mouse UCHL1 consisting of 233 a.a. and has a calculated molecular mass of 26.2 kDa. rm UCHL1 migrates as an approximately 29 kDa band in SDS-PAGE under reducing conditions. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Measured by the hydrolysis of UbiquitinAMC. The specific activity is >100 pmoles/min/ug. |
Formulation | Lyophilized from sterile 50mM Tris, 150mM NaCl, 20% glycerol, pH 7.7. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |