Name: Recombinant Mouse UCHL1 Protein, Active
Brand: Beta LifeScience
SKU: BL-4888PS
Availability: InStock

Catalog No.: BL-4888PS

Product Overview

Tag	N/A
Host Species	Mouse
Synonym	Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1,Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1.
Background	UbiquitinCarboxyl-Terminal Esterase L1 (UCHL1) is a part of a family whose productshydrolyze small C-terminal adducts of ubiquitin to create the ubiquitin monomer.UCHL1 is a part of the ubiquitin system, which regulates many biologicalactivities. UCHL1 is a thiol protease that distinguishes and hydrolyzes apeptide bond at the C-terminal glycine of ubiquitin. UCHL1 binds to free monoubiquitinand avoids its degradation in lysosomes.
Description	UCHL1 Mouse Recombinant expressed in E.Coli is a single, non-glycosylated polypeptide chain containing 246a.a. (1-223 a.a) and having a molecular weight of 27.2kDa.UCHL1 is fused to a 23a.a. His-tag at N-terminus and purified by unique purification methods.
Source	E.coli
AA Sequence	MGSSHHHHHH SSGLVPRGSH MGSMQLKPME INPEMLNKVL AKLGVAGQWR FADVLGLEEE TLGSVPSPAC ALLLLFPLTA QHENFRKKQI EELKGQEVSP KVYFMKQTIG NSCGTIGLIH AVANNQDKLE FEDGSVLKQF LSETEKLSPE DRAKCFEKNE AIQAAHDSVA QEGQCRVDDK VNFHFILFNN VDGHLYELDG RMPFPVNHGA SSEDSLLQDA AKVCREFTER EQGEVRFSAV ALCKAA.
Purity	Greaterthan 90.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Bioactivity	Specific activity is > 70 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.
Formulation	UCHL1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), 10% glycerol and 1mM DTT.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.