Recombinant Mouse TROP2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4674
Recombinant Mouse TROP2 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4674
Catalog No.: BLPSN-4674
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | Q8BGV3 |
| Synonym | C80403, EGP-1, GA733-1, Ly97, TROP2 |
| Background | TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. |
| Description | A DNA sequence encoding the extracellular domain of mouse TACSTD2 (Q8BGV3?) (Met 1-Gln 270) was fused with the Fc region of human IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gln 25 |
| AA Sequence | Met 1-Gln 270 |
| Molecular Weight | The secreted recombinant mouse TACSTD2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 487 a.a. and has a calculated molecular mass of 55 kDa. As a result of glycosylation, the apparent molecular mass of rmTACSTD2/Fc monomer is approximately 66 kDa in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.When 5 x 10E4 cells/well are added to coated plates (40 ug/mL, 100 uL/well), > 50% cells will adhere after 1 hour at 37°C. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
