Recombinant Mouse SFRP5 Protein (His tag)

Beta LifeScience SKU/CAT #: BLA-10087P

Recombinant Mouse SFRP5 Protein (His tag)

Beta LifeScience SKU/CAT #: BLA-10087P
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Product Overview

Host Species Mouse
Accession Q9WU66
Synonym Frizzled related protein 1b FRP 1b FRP1b SARP 3 SARP3 Secreted apoptosis related protein 3 Secreted frizzled related protein 5 SFRP 5
Description Recombinant Mouse SFRP5 Protein (His tag) was expressed in E.coli. It is a Full length protein
Source E.coli
AA Sequence APTRGQEYDYYGWQAEPLHGRSYSKPPQCLDIPADLPLCHTVGYKRMRLP NLLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVCLDRPIYPC RSLCEAARAGCAPLMEAYGFPWPEMLHCHKFPLDNDLCIAVQFGHLPATA PPVTKICAQCEMEHSADGLMEQMCSSDFVVKMRIKEIKIDNGDRKLIGAQ KKKKLLKAGPLKRKDTKKLVLHMKNGASCPCPQLDNLTGSFLVMGRKVEG QLLLTAVYRWDKKNKEMKFAVKFMFSYPCSLYYPFFYGAAEPH
Molecular Weight 37 kDa including tags
Purity >85% SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Liquid Solution
Stability The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution See related COA
Unit Definition For Research Use Only
Storage Buffer Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.
Subcellular Location Secreted.
Protein Families Secreted frizzled-related protein (sFRP) family
Database References

Gene Functions References

  1. Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle. PMID: 28287088
  2. SFRP5 be a target gene of PPARgamma. PMID: 29066306
  3. The balance of factors controlling fat deposition can be evaluated in part by the differential expression profiles of Mest and Sfrp5 genes with functions linked to fat deposition as long as there is an active accumulation of fat mass. PMID: 26001362
  4. the expression of Mest and Sfrp5 were tightly associated across the 5 mouse strains with the highest and lowest expression occurring in DBA/2J and C57BL/6J (B6) respectively suggesting a common mechanism for their regulation. PMID: 26005096
  5. although high sFRP5 expression inhibits B-lymphopoiesis in vivo, physiologically, it contributes to the preservation of very primitive lymphopoietic progenitors, including hematopoietic stem cells, under high estrogen levels PMID: 25676235
  6. A role for SFRP5 in glucose metabolism and pancreatic beta-cell function indicates that the use of an anti-SFRP5 mAb as a potential approach to treat type 2 diabetes. PMID: 25370851
  7. Sfrp5 mRNA expression and protein secretion depend on the differentiation of adipocytes. The dysregulation of Sfrp5 expression and secretion is directly correlated with insulin resistance. PMID: 22582808
  8. early progeny of Sox2-positive stem cells transiently expressed the Wnt inhibitor Sfrp5 PMID: 22819339
  9. SFRP5 inhibits WNT signaling to suppress oxidative metabolism and stimulate adipocyte growth during obesity. PMID: 22728933
  10. data show Sfrp5 is an anti-inflammatory adipokine whose expression is perturbed in models of obesity and type 2 diabetes; in obesity, Sfrp5 secretion exerts salutary effects on metabolic dysfunction by controlling adipose tissue inflammatory cells PMID: 20558665
  11. Here we report that the Secreted frizzled-related protein 5 (Sfrp5) gene, which encodes a secreted factor that can antagonize Wnt signaling, is expressed in the AVE and foregut endoderm during early mouse development PMID: 12972006
  12. Sfrp5 is not essential for axis formation or foregut morphogenesis in the mouse. Sfrp5 and Dkk1 together are not essential for anterior visceral endoderm development. PMID: 17133501
  13. Sfrp5 locally inhibits Wnt11 to maintain early foregut identity and to allow an epithelium to form over a mass of tissue undergoing Wnt-mediated cell movements. PMID: 18981481

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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