Recombinant Mouse S100A4 Protein

Name: Recombinant Mouse S100A4 Protein
Brand: Beta LifeScience
SKU: BLPSN-4124
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Mouse
Accession	P07091
Synonym	18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a
Background	S1A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S1 proteins containing two EF-hand calcium-binding motifs. In humans at least 2 S1 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S1A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S1A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin beta, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S1A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
Description	A DNA sequence encoding the mouse S100A4 (P07091)(Ala2-Lys101) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Source	HEK293
Predicted N Terminal	Gly
AA Sequence	Ala2-Lys101
Molecular Weight	The recombinant mouse S100A4 consists of 102 a.a. and predicts a molecular mass of 11.7 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
Purity	>85% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.