Recombinant Mouse RSPO1 Protein

Beta LifeScience SKU/CAT #: BL-4558PS
Recombinant Mouse RSPO1 Protein

Recombinant Mouse RSPO1 Protein

Beta LifeScience SKU/CAT #: BL-4558PS
Catalog No.: BL-4558PS

Product Overview

Tag His
Host Species Mouse
Synonym R-spondin-1, Cristin-3, mCristin-3, Roof plate-specificspondin-1, Rspo1, RSPO, R-spondin 1,R-spondin, Rspondin, CRISTIN3.
Background R-Spondin-1(Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator ofthe canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors.Rspo1 induces the onset of crypt cell proliferation and increases intestinalepithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway.
Description RSPO1 expressed in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 254a.a. (21-265a.a.) andhaving a molecular weight of 28.2kDa (Molecular size on SDS-PAGE will appear atapproximately 28-40kDa).RSPO1 is expressed with an 9a.a. His tag atC-Terminus and purified by unique purification methods.
Source Sf9
AA Sequence ADPSRGIKGKRQRRISAEGS QACAKGCELC SEVNGCLKCS PKLFILLERN DIRQVGVCLP SCPPGYFDAR NPDMNKCIKCKIEHCEACFS HNFCTKCQEG LYLHKGRCYP ACPEGSTAAN STMECGSPAQ CEMSEWSPWG PCSKKRKLCGFRKGSEERTR RVLHAPGGDH TTCSDTKETR KCTVRRTPCP EGQKRRKGGQ GRRENANRHP ARKNSKEPGSNSRRHKGQQQ PQPGTTGPLT SVGPTWAQHH HHHH.
Purity Greaterthan 90.0% as determined by SDS-PAGE.
Endotoxin <1.0 EU per μg by the LAL method.
Formulation RSPO1 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability Recombinant protein is stable for 12 months at -70°C
Usage For Research Use Only
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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