Recombinant Mouse RSPO1 Protein
Beta LifeScience
SKU/CAT #: BL-4558PS
Recombinant Mouse RSPO1 Protein
Beta LifeScience
SKU/CAT #: BL-4558PS
Catalog No.: BL-4558PS
Product Overview
| Tag | His |
| Host Species | Mouse |
| Synonym | R-spondin-1, Cristin-3, mCristin-3, Roof plate-specificspondin-1, Rspo1, RSPO, R-spondin 1,R-spondin, Rspondin, CRISTIN3. |
| Background | R-Spondin-1(Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator ofthe canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors.Rspo1 induces the onset of crypt cell proliferation and increases intestinalepithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway. |
| Description | RSPO1 expressed in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 254a.a. (21-265a.a.) andhaving a molecular weight of 28.2kDa (Molecular size on SDS-PAGE will appear atapproximately 28-40kDa).RSPO1 is expressed with an 9a.a. His tag atC-Terminus and purified by unique purification methods. |
| Source | Sf9 |
| AA Sequence | ADPSRGIKGKRQRRISAEGS QACAKGCELC SEVNGCLKCS PKLFILLERN DIRQVGVCLP SCPPGYFDAR NPDMNKCIKCKIEHCEACFS HNFCTKCQEG LYLHKGRCYP ACPEGSTAAN STMECGSPAQ CEMSEWSPWG PCSKKRKLCGFRKGSEERTR RVLHAPGGDH TTCSDTKETR KCTVRRTPCP EGQKRRKGGQ GRRENANRHP ARKNSKEPGSNSRRHKGQQQ PQPGTTGPLT SVGPTWAQHH HHHH. |
| Purity | Greaterthan 90.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | RSPO1 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
