Recombinant Mouse PPAR gamma Protein (His tag)

Beta LifeScience SKU/CAT #: BLA-10034P

Recombinant Mouse PPAR gamma Protein (His tag)

Beta LifeScience SKU/CAT #: BLA-10034P
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Product Overview

Host Species Mouse
Accession P37238
Synonym CIMT1 GLM1 NR1C3 Nuclear receptor subfamily 1 group C member 3 OTTHUMP00000185032 OTTHUMP00000185036 Peroxisome proliferator activated nuclear receptor gamma variant 1 Peroxisome Proliferator Activated Receptor gamma Peroxisome proliferator activated receptor gamma 1 Peroxisome proliferator-activated receptor gamma PPAR gamma PPAR-gamma PPARG PPARG_HUMAN PPARG1 PPARG2 PPARgamma
Description Recombinant Mouse PPAR gamma Protein (His tag) was expressed in E.coli. It is a Full length protein
Source E.coli
Molecular Weight 63 kDa including tags
Purity >85% SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Liquid Solution
Stability The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution See related COA
Unit Definition For Research Use Only
Storage Buffer Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.
Subcellular Location Nucleus. Cytoplasm.
Protein Families Nuclear hormone receptor family, NR1 subfamily
Database References
Tissue Specificity Highest expression in white and brown adipose tissue. Also found in liver, skeletal muscle, heart, adrenal gland, spleen, kidney and intestine. Isoform 2 is more abundant than isoform 1 in adipose tissue.

Gene Functions References

  1. these studies identify a PPARgamma-dependent miR-424/503-CD40 signaling axis that is critical for regulation of inflammation mediated angiogenesis PMID: 28566713
  2. WISP1 interacts with PPARgamma and that this interaction results in the inhibition of PPARgamma activity. T PMID: 28496206
  3. manipulation of PPAR-gamma activity has the potential to balance lipid-induced M1/M2 macrophage/Kupffer cell polarization. PMID: 28300213
  4. we identified IRF6 as a novel PPARgamma co-suppressor that serves a key role in suppressing PPARgamma-mediated cerebrovascular endothelial cytoprotection following ischemia. PMID: 28526834
  5. Data show that a peroxisome proliferator activated receptor gamma (PPARgamma)-dependent adipogenic response regulates muscle fat infiltration during regeneration. PMID: 30011852
  6. High fat diet-induced obesity exacerbates hematopoiesis deficiency and cytopenia caused by 5-fluorouracil via peroxisome proliferator-activated receptor gamma. PMID: 29305999
  7. findings suggest that MCAM is a gene upregulated and involved in maintaining PPARgamma induction in the late but not in the early stages of 3T3-L1 fibroblasts adipogenesis. PMID: 29468504
  8. Results suggest that hypothalamic peroxisome proliferator-activated receptor-gamma plays a vital role in ghrelin production and food intake in mice. PMID: 29655655
  9. Data suggest that expression of microRNA-128-3p is down-regulated during adipogenesis; an abundance of microRNA-128-3p appears to down-regulate adipogenesis and up-regulate lipolysis in adipocytes by targeting expression of Pparg (peroxisome proliferator-activated receptor gamma) and Sertad2 (SERTA domain-containing protein-2). PMID: 29654510
  10. The present study suggests for the first time that increased PPAR-gmma expression by high fat diet is responsible for cardiac dysfunction via upregulation of mitochondrial enzymes HMGCS2, BDH1 and PDK4. PMID: 30048968
  11. Data suggests that exposure to vitamin D deficiency during perinatal period directly affects expression of genes involved in development of adipose tissue in non-obese offspring; expression levels of Pparg (peroxisome proliferator activated receptor gamma) and Vdr (vitamin D receptor) are up-regulated in adipose tissue of male offspring. PMID: 28004271
  12. Our results found that, in the mice with T2D and AD, the activators of PPARg/AMPK signaling pathway significantly increased the expression level of IDE, and decreased the accumulation of Ab40 and Ab42, as well as alleviated the spatial learning and recognition impairments. PMID: 29222348
  13. PPARgamma functions as a checkpoint, guarding against inflammation, and is permissive for alternative activation of macrophages by facilitating glutamine metabolism PMID: 30006480
  14. Inhibition of this phosphorylation results in deregulation of p53 signaling, and biochemical studies show that PPARgamma physically interacts with p53 in a manner dependent on S273 phosphorylation. PMID: 29295932
  15. Direct regulation of mitochondrially encoded electron transport chain gene expression by mitochondrial PPARgamma2, in part, underlies the isoform-specific role for PPARgamma2 in brown adipocytes. PMID: 29566074
  16. L-Carnitine alleviated epithelial mesenchymal transformation-associated renal fibrosis caused by perfluorooctanesulfonate through a Sirt1- and PPARgamma-dependent mechanism. PMID: 28973641
  17. Pgc-1beta (-/-) hearts show pro-arrhythmic instabilities attributable to altered action potential conduction and activation rather than recovery characteristics. PMID: 28821956
  18. CACUL1 reciprocally regulates SIRT1 and LSD1 to repress PPARgamma and inhibit adipogenesis. PMID: 29233982
  19. results support a stimulatory effect of Pb on adipogenesis which involves ERK activation and C/EBPbeta upregulation prior to PPARgamma and adipogenesis activation. PMID: 28646352
  20. Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE(-/-) /LDLR(-/-) mouse via a PPARgamma/CD36 pathway. PMID: 28631352
  21. demonstrate that chronic ethanol ingestion activates peroxisome proliferator-activated receptor gamma (PPARgamma) and its target gene, monoacylglycerol O-acyltransferase 1 (MGAT1) PMID: 27404390
  22. The present results indicated that PPARgamma may serve a protective role on bEnd.3 cells and that BIRC5 may be a downstream target of PPARgamma regulation during cerebral ischemia. PMID: 29039513
  23. this study shows that Osterix represses adipogenesis by negatively regulating PPARgamma transcriptional activity PMID: 27752121
  24. HDAC3 inhibition in particular enhanced PPARgamma acetylation, prevented Klotho loss, and consequentially attenuated renal damage in mice model of chronic kidney disease. PMID: 28416226
  25. additional transgenic mouse PPAR-gamma or pharmacological activation of PPAR-gamma effectively prevented transgenic mouse DNMT1-induced proinflammatory cytokine production in macrophages and atherosclerosis development in the mouse model. PMID: 27530451
  26. data demonstrated that reduction of Pparg expression in T-helper cells is critical for spontaneous SLE-like autoimmune disease development; we also revealed a novel function of PPARgamma in lymphocyte trafficking and cross talk between Th17 and B cells. PMID: 27221351
  27. the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein alpha (C/EBPa), CCAAT enhancer-binding protein beta (C/EBPb), and gamma receptors by peroxisome proliferators (PPARg), during adipogenesis PMID: 28604636
  28. these findings identified an important role of renal tubular epithelium-targeted PPAR-gamma in maintaining the normal epithelial phenotype and opposing fibrogenesis, possibly via antagonizing oxidative stress PMID: 27602490
  29. Activation of PPARgamma in hematopoietic stem cells impaired hematopoietic repopulation. PPARgamma inhibition by shRNA or chemical compounds significantly improves the repopulating ability of Fancd2-/- HSCs. PMID: 28416286
  30. Study found that the metabolic master regulator PGC-1alpha is differentially affected by ALS-associated mutations in brain vs. peripheral tissues. Increased PGC-1alpha activity in peripheral tissue contributes to the metabolic phenotype, while in the CNS blunting of the PGC-1alpha response renders motoneurons vulnerable. PMID: 27818323
  31. DBZ is a putative PPARgamma agonist that prevents HFD-induced obesity-related metabolic syndrome and reverse gut dysbiosis. DBZ may be used as a beneficial probiotic agent to improve HFD-induced obesity-related metabolic syndrome in obese individuals PMID: 28736228
  32. Altogether, the authors demonstrate that Dnmt3a and Dnmt3b protect the epidermis from tumorigenesis and that squamous carcinomas are sensitive to inhibition of PPAR-gamma. PMID: 28425913
  33. FBXO9 directly interacted with PPAR gamma through the activation function-1 domain and ligand-binding domain. FBXO9 decreased the protein stability of PPAR gamma through induction of ubiquitination. PMID: 27197753
  34. inhibition of Hsp90 in Sec61a1 mutant hepatocytes also reduced Ppargamma protein levels and signaling. PMID: 24927728
  35. TET proteins, particularly TET2, were required for adipogenesis by modulating DNA methylation at the Ppargamma locus, subsequently by inducing Ppargamma gene expression. PMID: 28100914
  36. ATIP plays an important role in AT2 receptor-mediated PPARgamma activation. PMID: 26471325
  37. Our data showed that besides the high parasite burden and lack of microbicidal molecules, an imbalance with high COX-2 and 5-LOX eicosanoid expression and a lack of regulatory PPAR-gamma cytoplasm-to-nucleus translocation in macrophages were observed in mice that develop cerebral malaria. PMID: 27887739
  38. These results uncover a murine hepatic steatosis regulatory axis consisting of ABL1-PPARgamma2-MLL4, which may serve as a target of anti-steatosis drug development. PMID: 27806304
  39. Data suggest that a dietary factor, dietary supplement conjugated linoleic acid, improves endurance capacity of skeletal muscles independent of mild-intensity exercise/conditioning via Pparg-mediated mechanisms involved in gene expression regulation. PMID: 27736732
  40. madecassic acid was the active form of madecassoside in ameliorating colitis by restoring the Th17/Treg balance via regulating the PPARgamma/AMPK/ACC1 pathway. PMID: 28358365
  41. Pin1 enhances adipocyte differentiation by regulating the function of PPARgamma. PMID: 27475846
  42. The authors report that macrophage PPARgamma deletion in mice not only exacerbates mammary tumor development but also impairs the anti-tumor effects of rosiglitazone. Mechanistically, the authors identify Gpr132 as a novel direct PPARgamma target in macrophage whose expression is enhanced by PPARgamma loss but repressed by PPARgamma activation. PMID: 27692066
  43. Data indicate that obesity-induced insulin resistance and lipotoxicity can be treated with ginsenoside Rg3, which acts though the STAT5-PPAR gamma pathway in vivo and in vitro. PMID: 29042402
  44. TAK1 is required for PPARgamma transactivation and promotes PPARgamma transcriptional activity synergistically with TAK1 binding protein 1 (TAB1). PMID: 27293199
  45. PPAR gamma role in fat deposition and body weight gain.PPAR gamma is regulated by miR-27b. PMID: 28943435
  46. Time course analysis demonstrated that the adipogenic 'hub', sampled by PPARgamma and Lpin1, undergoes orchestrated reorganization during adipogenesis. PMID: 28755519
  47. Aleglitazar protects cardiomyocytes against hyperglycaemia-induced apoptosis by combined activation of both peroxisome proliferator-activated receptor-alpha and peroxisome proliferator-activated receptor-gamma. PMID: 28111985
  48. IRF6 suppresses PPARgamma through binding IRF recognition sites located upstream of the PPARgamma coding region. Taken together, the results suggest that an IRF6/PPARgamma regulatory axis suppresses anti-inflammatory responses in bone marrow-derived macrophages and provides references for future study addressing dysregulated metabolic and immunologic homeostasis of obese adipose tissue. PMID: 28645193
  49. adipogenic miR-27a in adipose tissue upregulates macrophage activation via inhibiting PPARgamma of insulin resistance induced by high-fat diet-associated obesity PMID: 28365247
  50. Report shows the identification of a novel Pparg splicing variant, Pparc1sv, in mice that is synergistically upregulated with Pparc2 during adipocyte differentiation of 3T3-L1 cells and mouse primary cultured preadipocytes. Both promotors are activated by C/EBPbeta and C/EBPdelta. PMID: 23840343


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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