Recombinant Mouse PF4 Protein

Beta LifeScience SKU/CAT #: BLA-10015P

Recombinant Mouse PF4 Protein

Beta LifeScience SKU/CAT #: BLA-10015P
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Product Overview

Host Species Mouse
Accession Q9Z126
Synonym C-X-C motif chemokine 4 Chemokine (C X C motif) ligand 4 Chemokine (CXC motif) ligand 4 chemokine ligand 4 CXCL 4 CXCL4 Iroplact MGC138298 Oncostatin A Oncostatin-A OncostatinA PF 4 PF-4 PF4 Pf4a Platelet factor 4 PLF4_HUMAN SCYB 4 SCYB4 short form Small inducible cytokine subfamily B Small inducible cytokine subfamily member 4
Description Recombinant Mouse PF4 Protein was expressed in E.coli. It is a Full length protein
Source E.coli
AA Sequence VTSAGPEESDGDLSCVCVKTISSGIHLKHITSLEVIKAGRHCAVPQLIAT LKNGRKICLDRQAPLYKKVIKKILES
Molecular Weight 8 kDa
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.
Formulation Lyophilised
Stability The recombinant protein samples are stable for up to 12 months at -80°C
Reconstitution See related COA
Unit Definition For Research Use Only
Storage Buffer Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Target Details

Target Function Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Database References

Gene Functions References

  1. these data demonstrate that PF4 has an important role in increasing B cell differentiation in the bone marrow environment PMID: 28914425
  2. these results identify CXCL4 as a vital immunoregulatory chemokine essential for protecting mice against influenza A virus infection, especially as it affects the development of lung injury and neutrophil mobilization to the inflamed lung PMID: 28120850
  3. These results indicate that CXCL4 is a novel Ni-binding protein that augments Ni allergy at the elicitation and sensitization phases. This is the first study to demonstrate that the Ni-binding protein augments Ni allergy in vivo. PMID: 28319310
  4. PF4 was produced by Ly6G+CD11b+ immature myeloid cells in the early stage premetastatic lungs, and decreased during metastatic progression. PMID: 27223426
  5. These data demonstrate that the CXCR2 network and CXCL4 play a role in the maintenance of normal HSC/HPC cell fates, including survival and self-renewal. PMID: 27222476
  6. Platelet-derived CXCL7 and CXCL4 contribute to the pathogenesis of acute lung injury. PMID: 27755915
  7. CXCL4 plays an important role in pancreatic inflammation PMID: 27183218
  8. Platelet secretion of CXCL4 is Rac1-dependent and regulates neutrophil infiltration and tissue damage in septic lung damage PMID: 26478565
  9. PF4 has a complex intramedullary life cycle with important implications in megakaryopoiesis and hematopoietic stem cell replication not seen with other tested alpha granule proteins. PMID: 26256688
  10. Heparin enhances antigen uptake and activation of the initial steps in the cellular immune response to PF4-containing complexes. PMID: 25960020
  11. Data indicate that platelet factor 4 (PF4) is involved directly in liver innate immune response against ischaemia-reperfusion injury (IRI) by regulating Th17 cell differentiation. PMID: 25440775
  12. CXCL4 regulates hematopoietic stem cell cell cycle activity. PMID: 25326802
  13. Platelet factor 4 has a role in regulating Th17 differentiation and cardiac allograft rejection PMID: 24463452
  14. Histones regulate activated protein C formation in a manner similar to PF4 and suggest heparinoids may be benificial in sepsis. PMID: 24177324
  15. PF4 drives a vascular smooth muscle inflammatory phenotype including a decline in differentiation markers, increased cytokine production, and cell proliferation. PMID: 23568488
  16. PF4 expression on intestinal epithelial cells is increased after IR at both the mRNA and protein levels. In conclusion, these findings demonstrate that may PF4 represent an important mediator of local and remote tissue damage. PMID: 22792197
  17. These results suggest that Cxcl4 and Cxcl7 play a role in the development of neurobehavioral alterations that are triggered by in utero TCDD exposure and later surface in adults. PMID: 21509788
  18. PF4 may have a role in bacterial defense, and heparin-induced thrombocytopenia is probably a misdirected antibacterial host defense mechanism. PMID: 20959601
  19. In vivo, half-life and diffusibility of CXCL4L1 compared with Cxcl4 is strongly increased. PMID: 20688960
  20. These data indicate that PF4 induction of monocyte KLF4 expression may be an important step in the pathogenesis of experimental cerebral malaria. PMID: 20454664
  21. Micronutrients effectively promote tumor dormancy in early prostate cancer by inducing platelet factor-4 expression and concentrating it at the tumor endothelium through enhanced platelet binding. PMID: 20525356
  22. CXC chemokine ligand 4 (Cxcl4) is a platelet-derived mediator of experimental liver fibrosis. PMID: 20162727
  23. important role for PF4 in thrombosis and neutralization of PF4 is an important component of heparin's anticoagulant effect. PMID: 14764524
  24. Platelet factor 4 and interleukin-8 CXC chemokine heterodimer formation modulates function at the quaternary structural level PMID: 15531763
  25. the PF4 storage pathway in alpha-granules is not a default pathway, but rather a regular granule storage pathway probably requiring specific sorting mechanisms PMID: 15613031
  26. analysis of granule targeting sequence within platelet factor 4 PMID: 15964840
  27. clinical heterogeneity in the HIT immune response may be due in part to requirements for specific biophysical parameters of the PF4/heparin complexes that occur in settings of intense platelet activation and PF4 release PMID: 17848616
  28. platelet-associated PF-4, but not its plasma counterpart, may represent a potential biomarker of early tumor presence PMID: 17914028
  29. platelet specific chemokine PF4 promotes atherosclerotic lesion development in vivo PMID: 18000617
  30. Brain microglia are a cellular source of CXCL4 gene expression. PMID: 18248618
  31. platelet-derived PF4 contributes to immune activation and T cell trafficking as part of the pathogenesis of experimental cerebral malaria . PMID: 18692777
  32. Platelet factor 4 regulates megakaryopoiesis through low-density lipoprotein receptor-related protein 1 (LRP1) on megakaryocytes. PMID: 19605848

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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