Recombinant Mouse ICAM2 Protein
Beta LifeScience
SKU/CAT #: BLPSN-2493
Recombinant Mouse ICAM2 Protein
Beta LifeScience
SKU/CAT #: BLPSN-2493
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | N/A |
| Host Species | Mouse |
| Accession | NP_034624.1 |
| Synonym | CD102, Icam-2, Ly-60 |
| Background | Intercellular adhesion molecule 2 (ICAM-2, CD12), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment. |
| Description | A DNA sequence encoding the mouse ICAM2 (NP_034624.1) (Met 1-Gln 222)was expressed with six amino acids (LEVLFQ) at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ser 20 |
| AA Sequence | Met 1-Gln 222 |
| Molecular Weight | The recombinant mouse ICAM2 consists of 210 a.a. and has a calculated molecular mass of 23.6 kDa. The recombinant protein migrates as an approximately 38-42 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10E4 cells/well are added to rmICAM1 coated plates (12.5 ug/mL with 100 uL/well), > 30% cells will adhere after 1 hour incubation at 37°C. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
