Recombinant Mouse Gremlin 1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2328
Recombinant Mouse Gremlin 1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2328
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | O70326 |
Synonym | Cktsf1b1, Drm, Grem, ld |
Background | GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon. |
Description | A DNA sequence encoding the mouse GREM1 (O70326) (Met1-Asp184) was fused with a His tag at the C-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Lys 25 |
AA Sequence | Met1-Asp184 |
Molecular Weight | The recombinant mouse GREM1 consists of 171 a.a. and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 1-7 ug/mL in the presence of 50 ng/mL of recombinant human BMP4. |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |