Recombinant Mouse gp130 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2302
Recombinant Mouse gp130 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2302
Catalog No.: BLPSN-2302
Product Overview
| Tag | His&Fc |
| Host Species | Mouse |
| Accession | NP_034690.3 |
| Synonym | 5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130 |
| Background | Glycoprotein 13 (also known as gp13, IL6ST, IL6-beta or CD13) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD13/gp13 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD13/gp13 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD13/gp13 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp13 for initiating signal transmission. CD13/gp13 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD13/gp13 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events. |
| Description | A DNA sequence encoding the mouse gp130 (NP_034690.3) extracellular domain (Met 1-Glu 617) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
| Source | CHO Cells |
| Predicted N Terminal | Glu 19 |
| AA Sequence | Met 1-Glu 617 |
| Molecular Weight | The recombinant mouse gp130/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 843 a.a. and has a predicted molecular mass of 94.7 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to inhibit the IL6 Ralpha enhancement of IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.03-0.15 ug/ml in the presence of 10 ng/ml recombinant human IL6sR and 20 ng/ml recombinant human IL6. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
