Recombinant Mouse GLIPR1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2261
Recombinant Mouse GLIPR1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2261
Catalog No.: BLPSN-2261
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_082884.1 |
| Synonym | 2410114O14Rik, mRTVP-1, RTVP-1, RTVP1 |
| Background | Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to theCRISP family. GLIPR1 / RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1 / RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1 / RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1 / RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1 / RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1 / RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1 / RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1 / RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1 / RTVP-1 is a potential therapeutic target in gliomas. |
| Description | A DNA sequence encoding the mouse GLIPR1 (NP_082884.1) (Met1-Thr223) was expressed with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Ser 18 |
| AA Sequence | Met1-Thr223 |
| Molecular Weight | The recombinant mouse GLIPR1 comprises 217 a.a. and has a predicted molecular mass of 25.1 kDa. The apparent molecular mass of the protein is approximately 28-32 kDa in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
