Recombinant Mouse GDF-8 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2224
Recombinant Mouse GDF-8 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2224
Catalog No.: BLPSN-2224
Product Overview
| Tag | Fc |
| Host Species | Mouse |
| Accession | NP_034964.1 |
| Synonym | Cmpt, Gdf8 |
| Background | GDF-8 / Myostatin / MSTN is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. GDF-8 / Myostatin / MSTN is highly expressed in skeletal muscle, and myostatin loss-of-function leads to doubling of skeletal muscle mass. Experiments in mice have improved that GDF-8 / Myostatin / MSTN is a key regulator of mesenchymal stem cell proliferation and differentiation, and mice lacking Myostatin encoding gene show decreased body fat and a generalized increase in bone density and strength. The increase in bone density is observed in most anatomical regions, including the limbs, spine, and jaw, and myostatin inhibitors have been observed to significantly increase bone formation. GDF-8 / Myostatin / MSTN is also expressed in the early phases of fracture healing, and GDF-8 / Myostatin / MSTN deficiency leads to increased fracture callus size and strength. Together, these data suggest that GDF-8 / Myostatin / MSTN has direct effects on the proliferation and differentiation of osteoprogenitor cells, and that GDF-8/Myostatin/MSTN antagonists and inhibitors are likely to enhance both muscle mass and bone strength. |
| Description | A DNA sequence encoding the mature form of mouse MSTN (NP_034964.1) (Asp 268-Ser 376) was fused with the Fc region of human IgG1 at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Glu 20 |
| AA Sequence | Asp 268-Ser 376 |
| Molecular Weight | The recombinant mouse MSTN/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 369 a.a. and has a predicted molecular mass of 40.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmMSTN/Fc monomer is approximately 45 kDa. |
| Purity | >92% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
