Recombinant Mouse Galectin-1 Protein

Name: Recombinant Mouse Galectin-1 Protein
Brand: Beta LifeScience
SKU: BLPSN-2191
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Mouse
Accession	P16045
Synonym	AA410090, Gal-1, Galbp, galectin-1, L-14.5, L14, Lect14
Background	Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.
Description	A DNA sequence encoding the mouse Lgals1 (P16045) (Met 1-Glu 135) was expressed and purified.
Source	E.coli
Predicted N Terminal	Met 1
AA Sequence	Met 1-Glu 135
Molecular Weight	The recombinant mouse Lgals1 consists of 135 a.a. and migrates as an approximately 15 kDa band as predicted in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 1-5 ug/ml.
Formulation	Lyophilized from sterile PBS, 100mM beta-Lactose, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Subcellular Location	Secreted, extracellular space, extracellular matrix. Cytoplasm. Secreted.
Database References	KEGG: mmu:16852 STRING: 10090.ENSMUSP00000086795 UniGene: Mm.43831

Gene Functions References

Overexpression of galectin-1 inhibited the proliferation of T-cells by means of HSC activation, which reduced the inflammatory response by exerting immunosuppressive effects and furthermore enhanced immune tolerance and alleviated hepatic fibrosis in liver transplantation. PMID: 30032146
Gal-1 is a major driver of pancreatic cancer progression. Genetic deletion of Gal1 in a Kras-driven mouse model of pancreatic ductal adenocarcinoma results in a significant increase in survival through mechanisms involving decreased stroma activation, attenuated vascularization, and enhanced T cell infiltration leading to diminished metastasis rates. PMID: 29615514
This work identified Gal1, an endogenous immune regulatory lectin, as an integral component of the secretory granule machinery and unveil the unexpected function of this lectin in regulating CTL killing activity. PMID: 29215607
The results of this study showed that development of spinal axons as well as the locomotor abilities observed in adult mice are independent of Gal-1. PMID: 28512739
GAL-1 also enhanced the generation of neural crest cells from explanted neural tubes PMID: 28695960
this present study indicated that Gal-1 secreted from MSCs upregulated expression of Gal-1 and stimulated formation of tolerance immunophenotype on DCs, where the underlying mechanism was the regulation of the MAPK signaling pathway in DCs, thereby inhibiting the function of DCs PMID: 28713822
markedly increased brain Gal-1 and S-nitrosylated Gal-1 both in scrapie-infected rodents and human prion diseases. PMID: 27211330
Gal-1 can limit eosinophil recruitment to allergic airways and suppresses airway inflammation by inhibiting cell migration and promoting eosinophil apoptosis. PMID: 27457925
Gal1 protein is essential for efficient liver regeneration following partial hepatectomy through the regulation of liver inflammation, hepatic cell proliferation, and the control of lipid storage in the regenerating liver. PMID: 27166189
findings show that galectin-1 (Gal-1), an immunoregulatory lectin widely expressed in mucosal tissues, contributes to Y. enterocolitica pathogenicity by undermining protective antibacterial responses. PMID: 28716827
Gal-1 functions as an important regulator in RNV and offers a promising strategy for the treatment of RNV diseases, such as proliferative diabetic retinopathy and retinopathy of prematurity. PMID: 28257831
These findings demonstrate that galectin-1 is required for i.v. tolerance induction, likely via induction of tolerogenic DCs leading to enhanced development of Tr1 cells, Treg cells, and downregulation of proinflammatory responses. PMID: 27151444
this study shows that galectin-1 secreted by Sertoli cells plays a pivotal role in the differentiation of functionally tolerogenic dendritic cells PMID: 26878424
analysis of the effect of low-temperature plasma on the expression of galectin-1, -2, and -3 in the healing skin compared with those of electrocoagulation conducted with a high-frequency electrical coagulator PMID: 26827730
Gal-1 does not play a role in the systemic immunosuppressive effect of Mesenchymal stem cells. PMID: 26857229
Galectin-1 has a role in driving lymphoma CD20 immunotherapy resistance in a mouse model PMID: 26888257
These findings highlight the importance of the inflammatory micro-environment of mucosal tissues in modulating intestinal epithelial cells susceptibility to the immunoregulatory lectin Gal-1 and its role in epithelial cell homeostasis. PMID: 26566180
results unveil a dual role of endogenous versus exogenous Gal-1 in the control of autoimmune testis inflammation. PMID: 26223819
Comparative transcriptome analyses identified Galectins 1 and 3 not only as markers in the lesioned adult cerebral cortex, but also as regulators of the proliferating and neurosphere-forming capacity of a subset of reactive astrocytes PMID: 26250529
GAL-1 is valuable biomarker of surgical stress after cardiac surgery. PMID: 26261609
The authors found that exposure of HL-1 cardiac cells to Gal-1 reduced the percentage of infection by two different Trypanosoma cruzi strains, Tulahuen (TcVI) and Brazil (TcI). PMID: 26451839
GAL1/pre-B-cell receptor interaction modifies GAL1/glycan affinity and particularly inhibits binding to LacNAc containing epitopes PMID: 25708191
Data indicate the relevance of the galectin-1 (Gal-1)-glycan axis in sperm function with implications in mammalian reproductive biology. PMID: 26136479
Galectin-1 accumulates in neurofilamentous lesions in SOD1(G93A) mice, as previously reported in humans with amyotrophic lateral sclerosis ( PMID: 24707896
it is shown that CRH and UCN upregulate galectin-1 expression in Ishikawa cell line and macrophages and this effect is mediated through CRHR1. PMID: 25473847
investigated the Citrobacter rodentium model of colonic infection and inflammation in Galectin-1 or Galectin-3 null mice PMID: 25243744
These findings define a novel role for galectin-1 in inhibiting tissue emigration of immunogenic, but not tolerogenic, dendritic cells, providing an additional mechanism by which galectin-1 can dampen immune responses. PMID: 26216879
These results suggested a novel role of inGal-1 in T-cells as a regulator of T-cell response to exGal-1, and its likely contribution to the mechanism in T-cell apoptosis deficiency in lupus. PMID: 25468561
These findings indicate that galectin-1 is a key regulator of wound repair that has therapeutic potential for pathological or imperfect wound healing. PMID: 25007042
Our results show that Gal-1, mainly in its dimeric form, promotes functional recovery of spinal lesions by interfering with inhibitory signals triggered by Sema3A binding to NRP-1/PlexinA4 complex. PMID: 24561343
These results underline the essential role of Gal-1 during endometriosis PMID: 24979200
Data show that plasma Galectin-1(GAL-1) level is significantly raised as early as 4 hours following myocardial infarction (MI). PMID: 24498007
Genetic ablation of Gal1 in a mouse model of pancreatic ductal adenocarcinoma dampened tumor progression by inhibiting proliferation, angiogenesis, desmoplasic reaction and by stimulating a tumor-associated immune response. PMID: 24812270
results indicate gal-1 is expressed in interneurons in the hippocampus that also express beta-tubulin III; findings suggest interneurons, which extend axons to form synapses, secrete gal-1 during neuronal pathfinding in the hippocampus PMID: 24323124
Galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation. PMID: 23954858
results strengthen the notion that Gal-1 is required for healthy gestation and highlight Gal-1 as a valuable biomarker for early PE diagnosis PMID: 23798433
Absence of endogenous Gal-1 results in an exacerbation of disease, in terms of both incidence and clinical severity. PMID: 23720814
Loss of Galectin-1 is associated with liver allograft rejection. PMID: 23356407
Galectin-1 controls cardiac inflammation and ventricular remodeling during acute myocardial infarction. PMID: 23142379
Endogenous expression of galectin-1 contributes to skin graft survival in the mouse. PMID: 22865279
These findings provide insights on the selective proapoptotic role of Gal-1 for a subpopulation of GABAergic interneurons PMID: 23115194
galectin-1 is one of the critical factors in MSCs regulating tumor progression PMID: 22844466
Galectin-1 is a pivotal regulator of M1 microglial activation that targets the activation of p38MAPK-, CREB-, and NF-kB-dependent signaling pathways and hierarchically suppresses downstream proinflammatory mediators, such as iNOS, TNF, and CCL2. PMID: 22884314
It was shown that the highly expressed galectin 1 in aggressive melanoma actively participates in metastasis. A role was shown for galectin 1 in the senescence of lung microvascular endothelial cells at the metastatic site. PMID: 22622427
galectin-1 inhibition reduces murine lung metastasis with increased CD4(+) and CD8 (+) T cells and reduced cancer cell adherence PMID: 22484915
gal-1 treatment represents a useful approach to control lesion severity in a virally induced immunopathological disease. PMID: 22467659
Gal-1 stimulated naive T cells, but had an antiproliferative effect when these cells were activated. When 2 Gal-1 molecules were covalently fused, the chimeric protein promoted proliferation suggesting that a tandem-repeat structure is required. PMID: 22357632
expression correlates with tumor growth rate, metastatic capacity and response to cyclophosphamide in T-cell lymphoma models PMID: 21947259
Lung cancer-derived galectin-1 enhances tumorigenic potentiation of tumor-associated dendritic cells by expressing heparin-binding EGF-like growth factor. PMID: 22291012
Have identified galectin-1 and galectin-3 as novel partners for VWF, and these proteins may modulate VWF-mediated thrombus formation. PMID: 22267483

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