Name: Recombinant Mouse Follistatin Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2123
Availability: InStock

Recombinant Mouse Follistatin Protein (His Tag)

Catalog No.: BLPSN-2123

Product Overview

Tag	His
Host Species	Mouse
Accession	P47931
Synonym	AL033346, FS
Background	Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 15 families, evidence was found for linkage between PCOS and follistatin. follistatin are expressed and subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal, bone marrow, and placenta but perhaps most notably in anterior pituitary-the classical target tissue for inhibin, the activin-follistatin system may play a key role in early embryogenesis. Follistatin binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone follistatin is a binding protein to activin. Since activin binds to follistatin, it is imperative to determine the nature of the activin/follistatin binding complex.
Description	A DNA sequence encoding the mouse FST (P47931) (Met 1-Trp 344) was expressed, fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Gly 30
AA Sequence	Met 1-Trp 344
Molecular Weight	The recombinant mouse FST comprises 326 a.a. and has a predicted molecular mass of 36.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFST is approximately 70-80 kDa due to glycosylation.
Purity	>98% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 0.02-0.08 ug/mL in the presence of 10 ng/ml Recombinant Human Activin A.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.