Recombinant Mouse FCGRT & B2M Heterodimer Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2009
Recombinant Mouse FCGRT & B2M Heterodimer Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-2009
Catalog No.: BLPSN-2009
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | P55899 |
| Synonym | FcRn |
| Description | A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of mouse FCGRT (P55899) was fused with a His tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified |
| Source | HEK293 |
| Predicted N Terminal | Ala 24 & Ile 21 |
| AA Sequence | Met 1-Met 119 |
| Molecular Weight | The recombinant heterodimer of mouse FCGRT / B2M comprises 385 ( 285 + 100 ) a.a. and has a calculated molecular mass of 43.5 ( 31.8 + 11.7 ) kDa. The apparent molecular mass of FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to bind human IgG1 in functional ELISA. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
