Recombinant Mouse FCGR4 Protein (His & AVI Tag), Biotinylated

Beta LifeScience SKU/CAT #: BLPSN-2003
Recombinant Mouse FCGR4 Protein (His & AVI Tag), Biotinylated

Recombinant Mouse FCGR4 Protein (His & AVI Tag), Biotinylated

Beta LifeScience SKU/CAT #: BLPSN-2003
Catalog No.: BLPSN-2003

Product Overview

Tag His&AVI
Host Species Mouse
Accession NP_653142.2
Synonym 4833442P21Rik, CD16-2, FcgammaRIV, Fcgr3a, FcgRIV, Fcrl3
Description The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal His tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Source HEK293
Predicted N Terminal Glu 19
AA Sequence Met 1-Gln 203
Molecular Weight The recombinant mouse FCGR4 consists of 214 a.a. and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 35-40 kDa due to glycosylation.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA.2. Labeling ratio of biotin to protein: 0.6
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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