Recombinant Mouse Ephrin B3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1881
Recombinant Mouse Ephrin B3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1881
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_031937.1 |
| Synonym | EFL-6, ELF-3, Elk-L3, Epl8, LERK-8, NLERK-2 |
| Background | Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons. |
| Description | A DNA sequence encoding the mouse Efnb3 (NP_031937.1) (Met1-Ala227) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Leu 28 |
| AA Sequence | Met1-Ala227 |
| Molecular Weight | The recombinant mouse Efnb3 consists 211 a.a. and predicts a molecular mass of 23.5 kDa. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | 1.Measured by its binding ability in a functional ELISA.2.Immobilized mouse EFNB3-His at 10ug/mL (100uL/well) can bind biotinylated mouse EPHB3-His, the EC50 of biotinylated mouse EPHB3-His 0.02-0.4 ug/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
