Recombinant Mouse EphB4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1844
Recombinant Mouse EphB4 Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1844
Collections: Enzymes, Kinase, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | Fc |
Host Species | Mouse |
Accession | P54761 |
Synonym | AI042935, Htk, MDK2, Myk1, Tyro11 |
Background | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
Description | A DNA sequence encoding the mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with the Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Leu 16 |
AA Sequence | Met 1-Ala 539 |
Molecular Weight | The secreted recombinant mouse EPHB4/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 765 a.a. and has a predicted molecular mass of 84.7 kDa. As a result of glycosylation, the apparent molecular mass of rm EPHB4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse EPHB2-His- at 10 ug/mL (100 ul/well) can bind mouse EPHB4-Fc, The EC50 of mouse EPHB4-Fc is 15 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |