Recombinant Mouse EphB2 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1834
Recombinant Mouse EphB2 Protein (Fc Tag)

Recombinant Mouse EphB2 Protein (Fc Tag)

Beta LifeScience SKU/CAT #: BLPSN-1834
Catalog No.: BLPSN-1834

Product Overview

Tag Fc
Host Species Mouse
Accession NP_034272.1
Synonym Cek5, Drt, Erk, ETECK, Hek5, Nuk, Prkm5, Qek5, Sek3, Tyro5
Background Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Description A DNA sequence encoding the extracellular domain of mouse EPHB2 (NP_034272.1) (Met1-Lys540) was expressed with the Fc region of human IgG1 at the C-terminus.
Source HEK293
Predicted N Terminal Val 19
AA Sequence Met1-Lys540
Molecular Weight The recombinant mouse EPHB2 comprises 760 a.a. and has a predicted molecular mass of 84.6 kDa. The apparent molecular mass of the protein is approximately 96.6 kDa in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse EFNB2-His at 10 ug/mL (100 uL/well) can bind mouse EPHB2-Fc. The EC50 of mouse EPHB2-Fc is 13.2-30.8ng/mL.
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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