Recombinant Mouse EphA1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1796
Recombinant Mouse EphA1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1796
Collections: Enzymes, Kinase, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | Q60750 |
| Synonym | 5730453L17Rik, AL033318, Eph, Esk |
| Background | EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. |
| Description | A DNA sequence encoding the extracellular domain of mouse EPHA1 (Q60750) (Met 1-Glu 548) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Arg 25 |
| AA Sequence | Met 1-Glu 548 |
| Molecular Weight | The secreted recombinant mouse EPHA1 comprises 535 a.a. and has a calculated molecular mass of 58.6 kDa. As a result of glycosylation, the apparent molecular mass of rmEPHA1 is approximately 68 kDa in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 ug/ml (100 ul/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
