Name: Recombinant Mouse ENTPD5 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1773
Availability: InStock

Recombinant Mouse ENTPD5 Protein (His Tag)

Catalog No.: BLPSN-1773

Product Overview

Tag	His
Host Species	Mouse
Accession	Q9WUZ9
Synonym	AI196558, AI987697, Cd39l4, ER-UDPase, mNTPase, NTPDase-5, NTPDase5, Pcph
Background	Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
Description	A DNA sequence encoding the mouse ENTPD5 (Q9WUZ9) (Thr19-Ser427) was fused with a His tag at the C-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Thr 19
AA Sequence	Thr19-Ser427
Molecular Weight	The recombinant mouse ENTPD5 consists of 419 a.a. and has a calculated molecular mass of 46.6 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to hydrolyze the 5-€™phosphate groups from the substrate guanosine 5-€™diphosphate (GDP).The specific activity is > 15000 pmols/min/ug.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.