Recombinant Mouse ENPP7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1765

Recombinant Mouse ENPP7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1765
Catalog No.: BLPSN-1765
Product Overview
Tag | His |
Host Species | Mouse |
Accession | Q3TIW9 |
Synonym | Alk-SMase, Gm254 |
Background | Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer. |
Description | A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ala 22 |
AA Sequence | Met 1-Gln 421 |
Molecular Weight | The secreted recombinant mouse ENPP7 consists of 411 a.a. and has a calculated molecular mass of 47 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions due to glycosylation. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |