Recombinant Mouse ENPP7 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1765
Recombinant Mouse ENPP7 Protein (His Tag)

Recombinant Mouse ENPP7 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1765
Catalog No.: BLPSN-1765

Product Overview

Tag His
Host Species Mouse
Accession Q3TIW9
Synonym Alk-SMase, Gm254
Background Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
Description A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Ala 22
AA Sequence Met 1-Gln 421
Molecular Weight The secreted recombinant mouse ENPP7 consists of 411 a.a. and has a calculated molecular mass of 47 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed