Recombinant Mouse E-Cadherin / ARC1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1680
Recombinant Mouse E-Cadherin / ARC1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1680
Catalog No.: BLPSN-1680
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | P09803 |
| Synonym | AA960649, ARC-1, E-cad, Ecad, L-CAM, Um, UVO |
| Background | Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas. |
| Description | A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Met 1-Val 709) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Glu 24 |
| AA Sequence | Met 1-Val 709 |
| Molecular Weight | The secreted recombinant mouse CDH1 (pro form) comprises 697 a.a. and has a calculated molecular mass of 78 kDa. As a result of different glycosylation, the apparent molecular mass of rm CDH1 is approximately 80-100 kDa in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10E4 cells/well are added to Recombinant Mouse Cadherin-1 coated plates (0.8 ug/mL with 100 uL/well), approximately >50% will adhere after 1 hour at 37°C. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
