Recombinant Mouse DLL4 Protein
Beta LifeScience
SKU/CAT #: BL-3771PS
Recombinant Mouse DLL4 Protein
Beta LifeScience
SKU/CAT #: BL-3771PS
Catalog No.: BL-3771PS
Product Overview
| Tag | His |
| Host Species | Mouse |
| Synonym | Delta-like protein 4, Drosophila Delta homolog 4, Delta 4, Dll4. |
| Background | Delta-Like4, also known as DLL4is implicated in the Notch signaling pathway as Notch ligand. Consequently DLL4negatively regulates endothelial cell proliferation, migration as well asangiogenic sprouting. DLL4 is vital for retinal progenitor proliferation andalso required for suppressing rod fates in late retinal progenitors and forproper generation of other retinal cell types. Furthermore, at some stage inthe spinal cord neurogenesis, DLL4 inhibits V2a interneuron fate. |
| Description | DLL4 expressed in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (27-532 a.a.) and fused to a 6 a.a. His Tag at C-terminus containing a total of 512a.a. and having a molecular weight of 55.8kDa.DLL4 shows multiple bands between 50-70kDa on SDS-PAGE, reducing conditions and purified by unique purification methods. |
| Source | Sf9 |
| AA Sequence | GSGIFQLRLQ EFVNQRGMLA NGQSCEPGCR TFFRICLKHF QATFSEGPCT FGNVSTPVLG TNSFVVRDKN SGSGRNPLQL PFNFTWPGTF SLNIQAWHTP GDDLRPETSP GNSLISQIII QGSLAVGKIW RTDEQNDTLT RLSYSYRVIC SDNYYGESCS RLCKKRDDHF GHYECQPDGS LSCLPGWTGK YCDQPICLSG CHEQNGYCSK PDECICRPGW QGRLCNECIP HNGCRHGTCS IPWQCACDEG WGGLFCDQDL NYCTHHSPCK NGSTCSNSGP KGYTCTCLPG YTGEHCELGL SKCASNPCRN GGSCKDQENS YHCLCPPGYY GQHCEHSTLT CADSPCFNGG SCRERNQGSS YACECPPNFT GSNCEKKVDR CTSNPCANGG QCQNRGPSRT CRCRPGFTGT HCELHISDCA RSPCAHGGTC HDLENGPVCT CPAGFSGRRC EVRITHDACA SGPCFNGATC YTGLSPNNFV CNCPYGFVGS RCEFPVGLPP SFPWVAHHHH HH |
| Purity | >95.0% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg by the LAL method. |
| Formulation | DLL4 protein solution (1mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability | Recombinant protein is stable for 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
