Recombinant Mouse DKK1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1619
Recombinant Mouse DKK1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1619
Catalog No.: BLPSN-1619
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | NP_034181.2 |
| Synonym | mdkk-1 |
| Background | Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers. |
| Description | A DNA sequence encoding the mouse DKK1 (NP_034181.2) (Met1-His272) was expressed with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Thr 32 |
| AA Sequence | Met1-His272 |
| Molecular Weight | The recombinant mouse DKK1 consists of 252 a.a. and predicts a molecular mass of 27.6 kDa. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Measured by its ability to inhibit recombinant rmWnt3a induced alkaline phosphatase production by C3H10T 1/2 cells. The ED50 for this effect is 0.05-0.3 ug/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
