Recombinant Mouse DDR1 Protein (His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-1570
Recombinant Mouse DDR1 Protein (His & GST Tag)

Recombinant Mouse DDR1 Protein (His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-1570
Catalog No.: BLPSN-1570

Product Overview

Tag His&GST
Host Species Mouse
Accession Q03146
Synonym 6030432F18, AI323681, Cak, CD167a, Nep, PTK3A
Background Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 1 (MCK1), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK1/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK1/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
Description A DNA sequence encoding the mouse DDR1 (Q03146-2) (Leu444-Val874) was fused with the N-terminal His-tagged GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Leu444-Val874
Molecular Weight The recombinant mouse DDR1/GST chimera consists of 668 a.a. and has a calculated molecular mass of 75.8kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity The specific activity was determined to be 2 nmol/min/mg using synthetic modified AXLtide peptide (modified-CKKSRGDYMTMQIG) as substrate.
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM DTT.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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