Recombinant Mouse CST6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1464

Recombinant Mouse CST6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1464
Catalog No.: BLPSN-1464
Product Overview
Tag | His |
Host Species | Mouse |
Accession | NP_082899.1 |
Synonym | 1110017E11Rik, ichq, N28197 |
Background | Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene. |
Description | A DNA sequence encoding the extracellular domain of mouse CST6 (NP_082899.1) (Met 1-Ala 152) was expressed, with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Glu 29 |
AA Sequence | Met 1-Ala 152 |
Molecular Weight | The secreted recombinant mouse CST6 comprises 132 a.a. and has a calculated molecular mass of 15.2 kDa. As a result of glycosylation, the apparent molecular mass of rmCST6 is approximately 20 kDa in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate ZFR-AMC,R&D Systems, Catalog # ES009.The IC50 is < 20 nM. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |