Recombinant Mouse CST6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1464
Recombinant Mouse CST6 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1464
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | NP_082899.1 |
Synonym | 1110017E11Rik, ichq, N28197 |
Background | Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene. |
Description | A DNA sequence encoding the extracellular domain of mouse CST6 (NP_082899.1) (Met 1-Ala 152) was expressed, with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Glu 29 |
AA Sequence | Met 1-Ala 152 |
Molecular Weight | The secreted recombinant mouse CST6 comprises 132 a.a. and has a calculated molecular mass of 15.2 kDa. As a result of glycosylation, the apparent molecular mass of rmCST6 is approximately 20 kDa in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate ZFR-AMC,R&D Systems, Catalog # ES009.The IC50 is < 20 nM. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |