Recombinant Mouse CES7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1225
Recombinant Mouse CES7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1225
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | NP_766347.1 |
Synonym | 1700081L16Rik, 1700122C07Rik, BB081581, cauxin, Ces7, Gm503 |
Background | Carboxylesterase belongs to the class of serine hydrolases family which includes Chymotrypsin and Acetylcholinesterase. Carboxylesterase is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Carboxylesterase is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it's widely distributed in cells especially in mammalian liver. Carboxylesterase 5 (CES5), also known as cauxin or CES7, is one of CES enzyme families exerting role in catalyzing hydrolytic and transesterfication reactions with broad substrat specifity. CES5 is reported in high concentrations in the urine of adult male cats, and within a protein complex of mammalian male epididymal fluids. Roles for carboxylesterase 5 may include regulating urinary levels of cat pheromone, catalyzing lipid transfer reactions within mammalian male reproductive fluids, and protecting neural tissue from drugs and xenobiotics. |
Description | A DNA sequence encoding the extracellular domain of mouse CES5 (NP_766347.1) (Met 1-His 556) was expressed, with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Gln 27 |
AA Sequence | Met 1-His 556 |
Molecular Weight | The secreted recombinant mouse CES5 comprises 541 a.a. and has a calculated molecular mass of 60.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions. |
Purity | >88% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >50,000 pmoles/min/ug. |
Formulation | Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |