Name: Recombinant Mouse CES7 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-1225
Availability: InStock

Recombinant Mouse CES7 Protein (His Tag)

Catalog No.: BLPSN-1225

Product Overview

Tag	His
Host Species	Mouse
Accession	NP_766347.1
Synonym	1700081L16Rik, 1700122C07Rik, BB081581, cauxin, Ces7, Gm503
Background	Carboxylesterase belongs to the class of serine hydrolases family which includes Chymotrypsin and Acetylcholinesterase. Carboxylesterase is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Carboxylesterase is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it's widely distributed in cells especially in mammalian liver. Carboxylesterase 5 (CES5), also known as cauxin or CES7, is one of CES enzyme families exerting role in catalyzing hydrolytic and transesterfication reactions with broad substrat specifity. CES5 is reported in high concentrations in the urine of adult male cats, and within a protein complex of mammalian male epididymal fluids. Roles for carboxylesterase 5 may include regulating urinary levels of cat pheromone, catalyzing lipid transfer reactions within mammalian male reproductive fluids, and protecting neural tissue from drugs and xenobiotics.
Description	A DNA sequence encoding the extracellular domain of mouse CES5 (NP_766347.1) (Met 1-His 556) was expressed, with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Gln 27
AA Sequence	Met 1-His 556
Molecular Weight	The secreted recombinant mouse CES5 comprises 541 a.a. and has a calculated molecular mass of 60.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.
Purity	>88% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >50,000 pmoles/min/ug.
Formulation	Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.