Recombinant Mouse Cathepsin L Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0606
Recombinant Mouse Cathepsin L Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0606
Catalog No.: BLPSN-0606
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | P06797 |
| Synonym | 1190035F06Rik, Ctsl1, fs, MEP, nkt |
| Background | Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. It is secreted by liver flukes at all stages of their development in the mammalian host, are believed to play important roles in facilitating parasite migration (tissue degradation), feeding and immuno-evasion. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. The enzyme takes part in nutrient acquisition by catabolizing host proteins to absorbable peptides, facilitates the migration of the parasite through the host intestine and liver by cleaving interstitial matrix proteins such as fibronectin, laminin and native collagen and is implicated in the inactivation of host immune defenses by cleaving immunoglobulins. Recently, Cathepsin L has been shown to suppress Th1 immune response in infected laboratory animals making them susceptible to concurrent bacterial infections. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a diagnostic or prognostic marker of human tumor malignancy. |
| Description | A DNA sequence encoding the mouse CTSL (P06797) (Met 1-Asn 334) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Thr 18 |
| AA Sequence | Met 1-Asn 334 |
| Molecular Weight | The secreted recombinant mouse CTSL (pro form) consists of 328 a.a. and has a calculated molecular mass of 37.3 kDa. The recombinant protein migrates as several bands probably including the pro form, mature form, the pro peptide and the cleaved heavy chain in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
