Recombinant Mouse Cathepsin A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0595
Recombinant Mouse Cathepsin A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0595
Catalog No.: BLPSN-0595
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | P16675 |
| Synonym | AU019505, PPCA, Ppgb |
| Background | Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation. |
| Description | A DNA sequence encoding the mouse CTSA (P16675-1) (Met 1-Tyr 474) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Ala 24 |
| AA Sequence | Met 1-Tyr 474 |
| Molecular Weight | The secreted recombinant mouse CTSA consists of 462 a.a. and has a calculated molecular mass of 52.8 kDa as estimated in SDS-PAGE under reducing conditions. |
| Purity | >96% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 25mM Tris, 0.3M NaCl, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
