Recombinant Mouse Carboxypeptidase B2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0572
Recombinant Mouse Carboxypeptidase B2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0572
Catalog No.: BLPSN-0572
Product Overview
| Tag | His |
| Host Species | Mouse |
| Accession | Q9JHH6 |
| Synonym | 1110032P04Rik, 4930405E17Rik, AI255929, CPR, Cpu, TAFI |
| Background | Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen. |
| Description | A DNA sequence encoding the extracellular domain of mouse CPB2 (Q9JHH6) (Met 1-Thr 422) was expressed, with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Phe 22 |
| AA Sequence | Met 1-Thr 422 |
| Molecular Weight | The secreted recombinant mouse CPB2 (pro form）comprises 412 a.a. and has a calculated molecular mass of 48 kDa. As a result of glycosylation, the apparent molecular mass of rmCPB2 is approximately 60 kDa in SDS-PAGE under reducing conditions. |
| Purity | >97% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
