Recombinant Mouse Carboxypeptidase B2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0572
Recombinant Mouse Carboxypeptidase B2 Protein (His Tag)

Recombinant Mouse Carboxypeptidase B2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0572
Catalog No.: BLPSN-0572

Product Overview

Tag His
Host Species Mouse
Accession Q9JHH6
Synonym 1110032P04Rik, 4930405E17Rik, AI255929, CPR, Cpu, TAFI
Background Carboxypeptidase B2, also known as Carboxypeptidase U, Thrombin-activable fibrinolysis inhibitor, Plasma carboxypeptidase B, CPB2, is a secreted protein which belongs to the peptidase M14 family. Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by thrombin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin / thrombomodulin complex. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. CPB2 exhibits carboxypeptidase activity and activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Description A DNA sequence encoding the extracellular domain of mouse CPB2 (Q9JHH6) (Met 1-Thr 422) was expressed, with a C-terminal His tag.
Source HEK293
Predicted N Terminal Phe 22
AA Sequence Met 1-Thr 422
Molecular Weight The secreted recombinant mouse CPB2 (pro form)comprises 412 a.a. and has a calculated molecular mass of 48 kDa. As a result of glycosylation, the apparent molecular mass of rmCPB2 is approximately 60 kDa in SDS-PAGE under reducing conditions.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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