Recombinant Mouse Carboxypeptidase A2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0567
Recombinant Mouse Carboxypeptidase A2 Protein (His Tag)

Recombinant Mouse Carboxypeptidase A2 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0567
Catalog No.: BLPSN-0567

Product Overview

Tag His
Host Species Mouse
Accession Q504N0
Synonym CPA2
Background Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Description A DNA sequence encoding the mouse CPA2 (Q504N0) (Met 1-Tyr 417) was expressed, with a C-terminal His tag.
Source HEK293
Predicted N Terminal Gln 17
AA Sequence Met 1-Tyr 417
Molecular Weight The secreted recombinant mouse CPA2 (pro form) comprises 412 a.a. and has a calculated molecular mass of 46.6 kDa. It migrates as an approximately 47 kDa in SDS-PAGE under reducing conditions.
Purity >92% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of DTNB.The specific activity is >4000 pmoles/min/ug.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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