Recombinant Mouse Carboxypeptidase A2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0567

Recombinant Mouse Carboxypeptidase A2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0567
Catalog No.: BLPSN-0567
Product Overview
Tag | His |
Host Species | Mouse |
Accession | Q504N0 |
Synonym | CPA2 |
Background | Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. |
Description | A DNA sequence encoding the mouse CPA2 (Q504N0) (Met 1-Tyr 417) was expressed, with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Gln 17 |
AA Sequence | Met 1-Tyr 417 |
Molecular Weight | The secreted recombinant mouse CPA2 (pro form) comprises 412 a.a. and has a calculated molecular mass of 46.6 kDa. It migrates as an approximately 47 kDa in SDS-PAGE under reducing conditions. |
Purity | >92% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of DTNB.The specific activity is >4000 pmoles/min/ug. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |